ABSTRACT
Objective:
Fibrosis around the dura is a concern in spinal surgery, affecting outcomes. Ligamentum flavum (LF) sparing and anti-adhesion barrier gel are potential strategies. This study aimed to compare LF-sparing and anti-adhesion barrier gels in reducing dural fibrosis in spinal surgery patients.
Materials and Methods:
Fifty patients underwent surgery at the thoracic/lumbar levels (T1 to S1). Twenty five received LF sparing and 25 received anti-adhesion barrier gels. Postoperative magnetic resonance imaging was performed 6 months post-surgery, using Ross classification for fibrosis assessment. Median fibrosis degree was computed, and surgical levels’ impact on fibrosis was analyzed.
Results:
The anti-adhesion barrier gel group had a higher mean age (53.68±7.54) than the LF-sparing group (48.36±8.03) (p<0.05, t-test). Gender distribution showed no significant difference (p=0.777, Chi-square test). The LF-sparing group had a lower mean degree of fibrosis (1.091.09±0.16) than the anti-adhesion barrier gel group (2.28±0.53) (p<0.001, Mann-Whitney U test). The LF sparing group’s mean fibrosis had no significant variance among spinal levels (p>0.05), while the anti-adhesion barrier gel group showed significant differences (p=0.004, p<0.01) with the highest fibrosis at L4-S1, followed by T12-L1 and L2-L3, and the lowest at T1-T11.
Conclusion:
LF sparing is more effective in reducing dural fibrosis than anti-adhesion barrier gel. Surgical level influence fibrosis, with LF sparing advantageous in the lower lumbar and thoracolumbar transitional zones.
INTRODUCTION
Spinal surgery is a common procedure for treating a variety of spinal disorders, including herniated discs, spinal stenosis, and spinal fractures. While the benefits of spinal surgery are well-established, the procedure also carries a risk of complications, including fibrosis around the dura. Fibrosis around the dura refers to the formation of scar tissue around the dura mater, which is the protective layer surrounding the spinal cord. This scar tissue can lead to nerve root compression, chronic pain, and other complications that can negatively impact patient outcomes.
To mitigate the risk of fibrosis around the dura, several techniques have been proposed, including ligamentum flavum (LF) sparing and the use of anti-adhesion barrier gels. The LF is a ligament located in the posterior spinal canal that is intimately associated with the dura mater. During spinal surgery, the LF can be thinned or completely removed to reduce the risk of fibrosis around the dura. Anti-adhesion barrier gels, on the other hand, are used to create a physical barrier between the dura mater and surrounding tissue, preventing the formation of scar tissue.
While both techniques have been shown to be effective in reducing the incidence of fibrosis around the dura, it is unclear which technique is more effective. Previous studies have reported conflicting results, with some studies suggesting that LF sparing is more effective than anti-adhesion barrier gel use, while others have found the opposite to be true(1-4). The purpose of this study is to compare the degree of fibrosis around the dura in patients undergoing spinal surgery with LF-sparing and anti-adhesion barrier gel use. By comparing the degree of fibrosis in these two groups, we can gain insight into the effectiveness of these techniques and potentially identify the best approach for reducing the incidence of fibrosis around the dura in spinal surgery patients
MATERIALS AND METHODS
The study was conducted between 2019 and 2022. A total of 50 patients were included in the study, with 25 patients undergoing LF sparing and 25 patients receiving anti-adhesion barrier gels during surgery. All patients underwent spinal surgery in the thoracic and lumbar region between T1 and S1 levels. Informed consent was obtained from our patients for our study. Institutional review board approval was obtained from the Ordu University Ethics Committee (approval number: 2023/167, date: 09.06.2023). In the ligament flavum sparing group, the ligament flavum was thinned and spared during surgery, while in the anti-adhesion barrier gel group, LF was totally removed and an anti-adhesion barrier gel was used to prevent adhesion formation. To provide detailed information on the degree of fibrosis in each patient, we evaluated the T1 and T2-weighted contrast-enhanced magnetic resonance imaging (MRI) assessments in relation to the extent of epidural fibrosis across five consecutive axial sections centered on the operated level. These sections were divided into four quadrants, with perpendicular lines drawn from the central aspect of the thecal sac as a reference. We graded the extent of fibrosis in each quadrant on a scale of 0 to 4, following Ross et al.’s(5) system. Grade 0 indicated no or minimal fibrosis, while Grade 4 indicated extensive fibrosis filling the quadrant. Fibrosis grades were recorded for each patient, considering four quadrants per section across the five sections. The median grade observed was used to determine the patient’s scarring grade. The patient characteristics, including age, sex, and surgical levels, were also recorded for each patient. To investigate whether the degree of fibrosis is influenced by the surgical level in addition to the applied surgical technique, we divided the patients into four groups based on the level of surgery: upper thoracic (T1-T11), transitional zone (T12-L1), upper lumbar (L2-L3), and lower lumbar (L4-S1). The statistics for each level were calculated separately.
Statistical Analysis
The data were analyzed using SPSS version 27.0. Descriptive data were expressed in mean ± standard deviation (SD), median (minimum-maximum) or number and frequency. The Shapiro-Wilk test was used to check normality of distribution of quantitative variables. The independent samples t-test was used to compare normally distributed quantitative variables, while the Mann-Whitney U test was used to compare non-normally distributed quantitative variables between the groups. Kruskal-Wallis test was used for the between group comparisons of parameters without normal distribution. Chi-square test was used to compare qualitative variables. A p-value less than 0.05 were considered statistically significant.
RESULTS
In a total of 50 cases; including 50% (n=25) female and 50% (n=25) male, the ages of the cases ranged from 35 to 68, with a median age of 50.0 and a mean age of 51.02±8.16 years. The mean degree of fibrosis ranged from 0.8 to 3.2, with a median value of 1.4 and a mean value of 1.69±0.71 (Table 1). In addition, the mean age was significantly higher in the anti-adhesion barrier gel group (mean=53.68, SD=7.54) compared to the LF sparing group (mean=48.36, SD=8.03) (p<0.05, t-test). There was no statistically significant difference in sex (p=0.777, chi-square test) between the two groups (Table 2).
The degree of fibrosis was significantly lower in the LF-sparing group (mean=1.09, SD=0.16) compared to the anti-adhesion barrier gel group (mean=2.28, SD=0.53) (p<0.001, Mann-Whitney U test). The difference in mean fibrosis between the two groups was statistically significant (Table 2) (Figure 1).
There was no statistically significant difference observed in the mean degree of fibrosis measurements among the spinal levels in the Ligament Flavum Sparing group (p>0.05), and in all levels, the degree of fibrosis was lower (Table 3). However, in the Anti-Adhesion Barrier Gel Use group, a statistically significant difference was found in the mean degree of fibrosis measurements among the spinal levels (p=0.004; p<0.01). The highest mean degree of fibrosis was observed in the L4-S1 (Lower Lumbar) level, followed by the T12-L1 (Transitional Zone) and L2-L3 (Upper Lumbar) levels. The lowest mean degree of fibrosis was found in the T1-T11 (Upper Thoracic) level (Table 4) (Figure 2).
DISCUSSION
The LF is a thin, fibrous band that helps to maintain spinal stability and runs between adjacent vertebrae. LF serves as an important barrier against connective tissue formation on the dura after surgery(3,6). During spinal surgery, the LF is often removed to access the spinal cord and other structures. However, recent studies have shown that preserving the LF during surgery can reduce the extent of fibrosis and improve surgical outcomes(7,8).
The present study investigated the degree of fibrosis around the dura in patients undergoing spinal surgery with ligament flavum sparing and anti-adhesion barrier gel use. Our results showed that the degree of fibrosis was significantly lower in the ligament flavum sparing group compared to the anti-adhesion barrier gel group, indicating that the use of ligament flavum sparing technique during spinal surgery may better reduce the degree of fibrosis around the dura.
These findings are in line with previous studies that have shown the benefits of LF sparing in reducing postoperative fibrosis and improving surgical outcomes(9,10). Some studies have demonstrated that patients who underwent surgery with LF preservation had a significantly lower incidence of postoperative fibrosis and lower back pain compared to those who underwent surgery without LF preservation(11,12).
On the other hand, there have been some conflicting findings in the literature regarding the effectiveness of anti-adhesion barrier gel in reducing fibrosis(13,14). The use of anti-adhesion barrier gel significantly showed that can reduce the incidence of postoperative fibrosis compared to the control group, suggesting that it can also be effective in reducing the degree of fibrosis around the dura(15).
Several techniques have been described for reducing postoperative fibrosis. A systematic review and meta-analysis by Hosseini et al.(16) found that the use of anti-adhesion barrier gel was effective in reducing epidural fibrosis. Another study also recommended one-day adhesiolysis to reduce the extent of epidural fibrosis with a significant success rate(17).
Wang et al.(18) evaluated the use of cross-linked hyaluronic acid gel in reducing epidural fibrosis after lumbar discectomy. Cross-linked hyaluronic acid gel was found to prevent epidural adhesion by inhibiting inflammatory factors and downregulating the expression of TGFb1 and COL1A1 mRNA, as demonstrated through in vivo and in vitro studies. This suggests that the use of an anti-adhesion barrier gel may have additional benefits beyond reducing fibrosis(18,19).
Various views have been proposed as to why connective tissue forms. Connective tissue formation may be a result of instability with age, sex, comorbidities, and axial predicates(20-22). Removal of the developing connective tissue may cause new adhesions, and its removal with a second operation poses a major problem, especially because of nerve damage and dural opening(23,24). Various suggestions on how to prevent connective tissue development have been reported. Among them are good control of wound bleeding, use of adhesion barriers agents, and recently, use of antifibrotic drugs(4,20,25,26). However, the most effective method is LF preservation. This structure has an important role in the biomechanics of the spinal region. It is helps stabilizes of the spinal column and prevents hypermobility by protecting the range of motion. It also supports elasticity and stability by being among the spinal posterior column elements. Recent studies have shown that preserving the LF during spinal surgery can help maintain the biomechanical stability of the spine and reduce the risk of complications such as hypermobility and adjacent segment degeneration. While preserving the LF may help maintain spinal stability, there is limited evidence to suggest that it can reduce the risk of hypermobility specifically. However, preserving the LF can help maintain the integrity of the posterior spinal ligaments and reduce the risk of iatrogenic injury to these structures, which can lead to instability and hypermobility. Additionally, preserving the LF may help maintain the biomechanical function of the spinal column, which can contribute to overall spinal stability(8,27,28).
Also, we identified a significant relationship between the type of surgical technique used, the level of spinal surgery, and the degree of fibrosis. In the case of using the anti-adhesion barrier gel, higher degrees of fibrosis were observed in the hypermobile spine regions, specifically the lower lumbar region and thoracolumbar transitional zone. This suggests that better control of fibrosis development, especially in hypermobile regions, can be achieved through the preservation of the LF. These findings are consistent with previous studies reporting higher degrees of fibrosis in the hypermobile region or in the presence of instability. Thoracic and lumbar and lumbosacral regions of the spine are subject to different biomechanical stresses compared to other spinal levels, which might influence the healing process and the development of fibrosis. Additionally, hypermobility itself can contribute to the formation of fibrosis. Excessive movement in the spinal segment can disrupt the delicate balance of tissue healing and increase the deposition of scar tissue. This can result in the formation of fibrotic adhesions around the spinal structures, including the epidural space(29,30).
The present study has some limitations, including a relatively small sample size and a lack of long-term follow-up data. However, further studies are required to identify the most effective strategies for preventing fibrosis, to determine the long-term effects of different surgical techniques on fibrosis and clinical outcomes in larger patient populations
In breaf, minimizing fibrosis formation is crucial in achieving successful spinal surgery outcomes, and recent literature suggests that the preservation of the LF and the use of minimally invasive surgical techniques may help reduce the extent of fibrosis and adhesion formation. Our study highlights the importance of using LF preserving technique that can reduce the degree of fibrosis around the dura during spinal surgery, particularly in the lower lumbar and thoracolumbar transitional region.
CONCLUSION
The use of the LF sparing technique can significantly reduce the degree of fibrosis around the dura compared to the use of anti-adhesion barrier gel. This reduction in fibrosis may result in improved patient outcomes and a decreased incidence of nerve root compression and chronic pain. Additionally, the level of surgery plays a crucial role in the development of fibrosis around the dura. To select the most effective surgical techniques for minimizing postoperative fibrosis, it is better to utilize the LF sparing technique rather than anti-adhesion barrier gels, particularly in the lower lumbar region and thoracolumbar transitional zone.
